human bone marrow-derived mesenchymal stem cell line hbmsc  (Procell Inc)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: PTBP1 as a Promising Predictor of Poor Prognosis by Regulating Cell Proliferation, Immunosuppression, and Drug Sensitivity in SARC

doi: 10.1155/2022/5687238

Figure Lengend Snippet: Clinical and vitro validation of PTBP1 in osteosarcoma. (a) Kaplan-Meier analysis of OS in TARGET osteosarcoma patients stratified by different expression levels of PTBP1. (b) Representative images of IHC staining of PTPB1 in human osteosarcoma tumors and adjacent tissues. Scale bar: 200/50 μ m. IHC score: 0, negative; 1, weakly positive; 2 moderately positive; 3 strongly positive staining. (c) Barplot of IHC score for PTBP1 protein level assessed by IHC assay. (d) Western blotting of PTBP1 protein expression in human osteosarcoma cell lines and hBMSC cell line. (e) RT-PCR result of PTBP1 gene expression in HOS-WT and HOS-PTBP1-KO cell line. (f) Western blotting of PTBP1 protein expression in HOS-WT and HOS-PTBP1-KO cell line. (g) Cell proliferation of HOS-WT and HOS-PTBP1-KO cell line. (h) Cell migration and invasion of HOS-WT and HOS-PTBP1-KO cell line. ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, scale bar: 100 μ m.

Article Snippet: Human bone marrow stromal (mesenchymal) stem cell line hBMSC and human osteosarcoma cell lines (HOS, MG63, Saos2, and U2OS) originated from American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Migration